News

CompCancer Seminar 06.12.2023 - Dana Pe’er

 

For the seminar on the 6th of December Sofya Marchenko invited Dana Pe’er from the Sloan Kettering Institute (New York, USA), to give us a talk on her recent work. If you are interested in joining write an e-Mail to compcancer at charite dot de to receive the zoom link.

Because of the time difference, the literature seminar will take place virtually on zoom at 3 pm (Berlin- time).

 

Dr. Dana Pe’er is chair and professor in Computational and Systems Biology Program at Sloan Kettering Institute and a researcher in computational systems biology. She was previously a professor at columbia  Department of Biological Sciences. Pe’er’s research focuses on understanding the organization, function and evolution of molecular networks, particularly how genetic variations alter the regulatory network and how these genetic variations can cause cancer. Dana Pe’er investigates cellular development – in particular, how healthy cells shape their identities and build and repair organs, how cancer cells abandon those identities and hijack those processes, and how tissue environments influence an individual cell’s fate. Pe’er’s lab combines mathematical approaches with a range of emerging technologies that enable large-scale analyses of single cells. They are developing computational frameworks to characterize cell-state compositions and tissue-level dynamics and seeking to answer a variety of questions related to the causes and consequences of plasticity in development, regeneration, and cancer. For example, Pe’er wants to understand intratumoral heterogeneity which is essential for predicting cancer behavior and identifying molecular vulnerabilities.

Her Talk is titled „Beyond Cells: Gene programs and tissue context”. In which she will among others talk about methods and results from her very recent publication in Nature Biotechnology „Supervised discovery of interpretable gene programs from single-cell data” (https://www.nature.com/articles/s41587-023-01940-3).

 

 

CompCancer Seminar 10.08.2023 - Niklas J. Lang

Our PhD student Karina Tristan invited Niklas J. Lang (MD candidate) to present at the CompCancer Literature Seminar, which will take place Thursday, 10th August, at 10 am.

 

It will be held in person at:

Maud Menten Hall

Philippstraße 13, Haus 18

10115 Berlin, Germany

[Google Maps link: https://goo.gl/maps/9zvz8Z48oTstZFjZ9]

If you are interested in joining online write an e-Mail to compcancer at charite dot de to receive the zoom link.

 

Paper: Ex vivo tissue perturbations coupled to single cell RNA-seq reveal multi-lineage cell circuit dynamics in human lung fibrogenesis
https://www.biorxiv.org/content/10.1101/2023.01.16.524219v1.full 

ABSTRACT
Pulmonary fibrosis develops as a consequence of failed regeneration after injury. Analyzing mechanisms of regeneration and fibrogenesis directly in human tissue has been hampered by the lack of organotypic models and analytical techniques. In this work, we coupled ex vivocytokine and drug perturbations of human precision-cut lung slices (hPCLS) with scRNAseq and induced a multi-lineage circuit of fibrogenic cell states in hPCLS, which we show to be highly similar to the in vivo cell circuit in a multi-cohort lung cell atlas from pulmonary fibrosis patients. Using micro-CT staged patient tissues, we characterized the appearance and interaction of myofibroblasts, an ectopic endothelial cell state and basaloid epithelial cells in the thickened alveolar septum of early-stage lung fibrosis. Induction of these states in the ex vivo hPCLS model provides evidence that the basaloid cell state was derived from alveolar type-2 cells, whereas the ectopic endothelial cell state emerged from capillary cell plasticity. Cell-cell communication routes in patients were largely conserved in the hPCLS model and anti-fibrotic drug treatments showed highly cell type specific effects. Our work provides an experimental framework for perturbational single cell genomics directly in human lung tissue that enables analysis of tissue homeostasis, regeneration and pathology. We further demonstrate that hPCLS offers novel avenues for scalable, high-resolution drug testing to accelerate anti-fibrotic drug development and translation.



Niklas Lang's Bio:

Mr. Niklas Lang is a final-year medical student at LMU Munich (GER). He obtained his M.Sc. in Bioinformatics from the University of Edinburgh (UK) in 2020. He is now an MD candidate in the Lab of Dr. Herbert Schiller at the Institute of Lung Health and Immunity at Helmholtz Munich and the Comprehensive Pneumology Center of the LMU Klinikum. His research interest lies in understanding the spatiotemporal dynamics of cell circuits that orchestrate lung fibrogenesis through the lens of single-cell genomics.

 

 

CompCancer continues!

We are very pleased to announce the prolongation of our graduate programme CompCancer for a second funding period!

DFG press release from Mai 8th

This also means soon we will recruit new PhD students for more interesting research projects!

Stay tuned and sign up for our mailing list.

 

 

New Mailing List

We have a new mailing list for people who are interested in our PhD programme and events that are open to everyone (such as our colloquium).

Please join here

Join and stay updated!

 

 

Pint of Science Festival- May 22-24

This year we support the Pint of Science festival

Listen to scientists talking about their research in a fun and easily understandable way while enjoying a pint of beer!

Everyone can be involved, ask questions and discuss interesting topics of all areas of science.

This year, the events will be on Monday, 22nd May at Volksbar (German), on Tuesday, 23rd May at Kohlenquelle (English) and Wednesday, 24th May at Kallasch (English).


You need to buy tickets, (2.50€). Be quick as the events are usually sold out!

Looking forward to seeing many of you there!

 

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About

The research training group CompCancer (RTG2424) is a DFG funded PhD programme in Berlin, focussing on computational aspects of cancer research.

Contact: compcancer at charite dot de